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The WinCF Model – An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections
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JoVE 杂志 免疫与感染
The WinCF Model – An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections

The WinCF Model – An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections

8,968 Views

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06:57 min

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May 08, 2017

DOI:

10.3791/55532-v

DOI:
10.3791/55532-v

•

06:57 min
May 08, 2017

•

3 Views
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1Skaggs School of Pharmacy and Pharmaceutical Sciences,University of California, San Diego, 2Department of Mechanical and Aerospace Engineering,University of California, San Diego

Summary

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The mucus plugged airways of cystic fibrosis (CF) patients are an ideal environment for microbial pathogens to thrive. The manuscript describes a novel method for studying the CF lung microbiome in an environment that mimics where they cause disease and how alterations of chemical conditions can drive microbial dynamics.

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Cite this Article

Comstock, W. J., Huh, E., Weekes, R., Watson, C., Xu, T., Dorrestein, P. C., Quinn, R. A. The WinCF Model - An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections. J. Vis. Exp. (123), e55532, doi:10.3791/55532 (2017).

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