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Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
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Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
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January 07, 2019
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Here, we describe a complete workflow for the qualitative and quantitative analysis of immune synapses between primary human T cells and antigen-presenting cells. The method is based on imaging flow cytometry, which allows the acquisition and evaluation of several thousand cell images within a relatively short period of time.
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Wabnitz, G., Kirchgessner, H., Samstag, Y. Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry. J. Vis. Exp. (143), e55345, doi:10.3791/55345 (2019).
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