/
/
Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
11,079 Views
•
08:35 min
•
January 07, 2019
DOI:
Summary
Automatically generated
Here, we describe a complete workflow for the qualitative and quantitative analysis of immune synapses between primary human T cells and antigen-presenting cells. The method is based on imaging flow cytometry, which allows the acquisition and evaluation of several thousand cell images within a relatively short period of time.
Read Article
Cite this Article
Wabnitz, G., Kirchgessner, H., Samstag, Y. Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry. J. Vis. Exp. (143), e55345, doi:10.3791/55345 (2019).
Copy