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使用主神经球文化研究纤毛
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Using Primary Neurosphere Cultures to Study Primary Cilia
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使用主神经球文化研究纤毛

DOI:
10.3791/55315-v

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08:14 min

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April 14, 2017

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, , ,
1Department of Cell Biology,University of Texas Southwestern Medical Center

Capitoli

  • 00:05Titolo
  • 01:13Isolation of Neurospheres from the Adult Mouse Brain
  • 02:43Embedding and Sectioning of Neurospheres for immunostaining
  • 04:10Starvation and Analysis of Cilia
  • 06:01Results: Starvation Induces Ciliogenesis in Dissociated, Adherent Neutral Stem/Progenitor Cells
  • 07:19Conclusion

Summary

Traduzione automatica

Traduzione automatica

初级纤毛是在神经祖细胞增殖，神经细胞的分化，和成人神经元功能至关重要的。这里，我们描述研究ciliogenesis和信号蛋白使用初级神经球培养物在神经干/祖细胞和分化的神经元纤毛的贩卖的方法。

Tags

Keywords: Primary Neurosphere CulturesPrimary CiliaNeural Stem CellsNeural ProgenitorsNeuronsLateral VentricleSubventricular ZoneTrypsin-EDTABasal MediumNeurospheresImmunostainingParaformaldehydeSucrose

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