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在缺血样条件下监测星形胶质细胞的反应性和增殖体外
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Neuroscienze
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JoVE Journal Neuroscienze
Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
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在缺血样条件下监测星形胶质细胞的反应性和增殖体外

9,632 Views

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15:08 min

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October 21, 2017

DOI:

10.3791/55108-v

DOI:
10.3791/55108-v

•

15:08 min
October 21, 2017

•

3 Views
, , , ,
1Department of Neuroscience, School of Medicine,Universidad Central del Caribe, 2Department of Biochemistry, School of Medicine,Universidad Central del Caribe, 3Department of Pharmacology and Toxicology, Medical Sciences, Campus,University of Puerto Rico

Summary

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缺血性中风是一个复杂的事件, 其中星形胶质细胞对受氧葡萄糖剥夺 (OGD) 影响的脑区的具体贡献是很难研究的。本文介绍了一种获取孤立星形胶质细胞的方法, 并研究了其在 OGD 条件下的反应性和增殖。

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Ferrer-Acosta, Y., Gonzalez-Vega, M. N., Rivera-Aponte, D. E., Martinez-Jimenez, S. M., Martins, A. H. Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions. J. Vis. Exp. (128), e55108, doi:10.3791/55108 (2017).

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