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JoVE Journal Chemistry
In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
 

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Article DOI: 10.3791/55041-v • 14:54 min • February 3rd, 2018
February 3rd, 2018 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 1,2
1Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Université de Bordeaux, 2Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), CNRS, 3Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB), CNRS, 4Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB), Université de Bordeaux
* These authors contributed equally

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We describe a procedure for the detection of chemical elements present in situ in human cells as well as their in vitro quantification. The method is well-suited to any cell type and is particularly useful for quantitative chemical analyses in single cells following in vitro metal oxide nanoparticles exposure.

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In Situ Detection Single Cell Quantification Metal Oxide Nanoparticles Nuclear Microprobe Analysis Nanoparticle Exposure Human Health Risks Behavior Of Nanoparticles Metabolic Responses Detection Methods Quantification Methods Internalized Nanoparticles Analytical Tools Microscopy Mass Spectrometry Atomic Spectroscopy Correlative Approach Fluorescent Tag Fluorescence Microscopy Nuclear Microprobe Analysis Cellular Density Chemical Element Distribution Nanoparticles Per Cell Titanium Dioxide Nanoparticles
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