Rivista
/
Genetica
/
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
JoVE Journal
Genetica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Genetica
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

8,846 Views

•

10:06 min

•

April 26, 2017

DOI:

10.3791/54967-v

DOI:
10.3791/54967-v

•

10:06 min
April 26, 2017

•

5 Views
, , , , , ,
1Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology,Shanghai Institutes for Biological Sciences (SIBS), 2Institute of Cancer Stem Cell, Second Affiliated Hospital, Cancer Center,Dalian Medical University

Summary

Automatically generated

This report describes a bioengineering method to design and construct novel Artificial Splicing Factors (ASFs) that specifically modulate the splicing of target genes in mammalian cells. This method can be further expanded to engineer various artificial factors to manipulate other aspects of RNA metabolism.

Explore More Videos

Alternative SplicingArtificial Splicing FactorsPUF DomainRNA ProcessingHEK-293T CellsTransfectionSplicing Reporter PlasmidsGlycine-rich DomainRS-effector DomainPGL-RS-PUF Expression Vector

Read Article

Cite this Article

Wei, H., Liu, Y., Wang, Y., Lu, Q., Yang, X., Li, J., Wang, Z. Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells. J. Vis. Exp. (122), e54967, doi:10.3791/54967 (2017).

Download .ris file

Copy

Share Video

.

Copy