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Optogenética aleatoria de mutagénesis, utilizando la histona-miniSOG en<em> C. elegans</em
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Genetica
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JoVE Journal Genetica
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
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Optogenética aleatoria de mutagénesis, utilizando la histona-miniSOG en<em> C. elegans</em

8,880 Views

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04:51 min

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November 14, 2016

DOI:

10.3791/54810-v

DOI:
10.3791/54810-v

•

04:51 min
November 14, 2016

•

2 Views
,
1Division of Biological Sciences, Section of Neurobiology,University of California in San Diego and Howard Hughes Medical Institute, 2Department of Cellular and Molecular Medicine,University of California in San Diego School of Medicine and Howard Hughes Medical Institute

Summary

Automatically generated

codificado genéticamente-histona-miniSOG induce mutaciones hereditarias en todo el genoma de una manera dependiente de la luz azul. Este método de mutagénesis es simple, rápido, libre de productos químicos tóxicos y muy adecuado para la detección genética hacia adelante y la integración del transgén.

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Cite this Article

Noma, K., Jin, Y. Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans. J. Vis. Exp. (117), e54810, doi:10.3791/54810 (2016).

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