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でヒストンminiSOGを使用した光遺伝学ランダム突然変異誘発<em> C。エレガンス</em
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Genetica
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JoVE Journal Genetica
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
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でヒストンminiSOGを使用した光遺伝学ランダム突然変異誘発<em> C。エレガンス</em

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04:51 min

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November 14, 2016

DOI:

10.3791/54810-v

DOI:
10.3791/54810-v

•

04:51 min
November 14, 2016

•

2 Views
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1Division of Biological Sciences, Section of Neurobiology,University of California in San Diego and Howard Hughes Medical Institute, 2Department of Cellular and Molecular Medicine,University of California in San Diego School of Medicine and Howard Hughes Medical Institute

Summary

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遺伝的にコードされたヒストンminiSOGは、青色光依存的にゲノムワイドな遺伝変異を誘発します。この突然変異誘発法は、高速で、単純な有毒化学物質の自由、およびフォワード遺伝子スクリーニングおよび導入遺伝子組込みに適しています。

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Cite this Article

Noma, K., Jin, Y. Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans. J. Vis. Exp. (117), e54810, doi:10.3791/54810 (2016).

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