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光遗传学随机突变用组蛋白miniSOG在<em>℃。线虫</em
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Genetica
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JoVE Journal Genetica
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
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光遗传学随机突变用组蛋白miniSOG在<em>℃。线虫</em

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04:51 min

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November 14, 2016

DOI:

10.3791/54810-v

DOI:
10.3791/54810-v

•

04:51 min
November 14, 2016

•

2 Views
,
1Division of Biological Sciences, Section of Neurobiology,University of California in San Diego and Howard Hughes Medical Institute, 2Department of Cellular and Molecular Medicine,University of California in San Diego School of Medicine and Howard Hughes Medical Institute

Summary

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基因编码的蛋白，miniSOG诱导蓝光依赖性全基因组的遗传突变。这种突变方法简单，快速，免费的有毒化学物质，并非常适用于正向遗传学筛查和转基因整合。

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Cite this Article

Noma, K., Jin, Y. Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans. J. Vis. Exp. (117), e54810, doi:10.3791/54810 (2016).

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