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Preparación de la matriz extracelular fibras de proteína de Brillouin Spectroscopy
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
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Preparación de la matriz extracelular fibras de proteína de Brillouin Spectroscopy

9,989 Views

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07:19 min

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September 15, 2016

DOI:

10.3791/54648-v

DOI:
10.3791/54648-v

•

07:19 min
September 15, 2016

•

5 Views
, , , , , ,
1School of Physics and Astronomy,University of Exeter, 2Department of Physics and Geology,University of Perugia, 3Istituto Officina dei Materiali del CNR,Unità di Perugia

Summary

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We present a protocol for the application of Brillouin light scattering spectroscopy to elastin and trypsin-purified type I collagen fibers of the extracellular matrix to extract their full elastic properties.

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Cite this Article

Edginton, R. S., Mattana, S., Caponi, S., Fioretto, D., Green, E., Winlove, C. P., Palombo, F. Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy. J. Vis. Exp. (115), e54648, doi:10.3791/54648 (2016).

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