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Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina
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Neuroscienze
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JoVE Journal Neuroscienze
Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina

Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina

8,059 Views

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08:44 min

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October 13, 2016

DOI:

10.3791/54608-v

DOI:
10.3791/54608-v

•

08:44 min
October 13, 2016

•

4 Views
, , ,
1Department of Ophthalmology,Baylor College of Medicine, 2Department of Neuroscience,Baylor College of Medicine, 3Department of Biochemistry and Molecular Biology,Baylor College of Medicine

Summary

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This protocol demonstrates how to perform whole-cell patch clamp recording on retinal neurons from a flat-mount preparation.

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Patch ClampStarburst Amacrine CellsFlat-mountDeafferentated Mouse RetinaRetinal NeurobiologyRetinal CircuitsWhole-cell RecordingNitrocellulose MembraneExternal SolutionCarbogenatedUpright Microscope

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Tu, H., Hsu, C., Chen, Y., Chen, C. Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina. J. Vis. Exp. (116), e54608, doi:10.3791/54608 (2016).

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