Journal
/
Neurowissenschaften
/
Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina
JoVE Journal
Neurowissenschaften
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Neurowissenschaften
Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina

Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina

DOI:
10.3791/54608-v

•

08:44 min

•

October 13, 2016

•

, , ,
1Department of Ophthalmology,Baylor College of Medicine, 2Department of Neuroscience,Baylor College of Medicine, 3Department of Biochemistry and Molecular Biology,Baylor College of Medicine

Kapitel

  • 00:05Titel
  • 01:03Retina Dissection
  • 03:30Whole-cell Patch Clamp Recording from Flat-mount Retina
  • 06:34Results: Morphology and Membrane Potentials of ON-and Off-Starburst Amacrine Cell in the Nob Mouse Retina
  • 07:19Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol demonstrates how to perform whole-cell patch clamp recording on retinal neurons from a flat-mount preparation.

Tags

Patch ClampStarburst Amacrine CellsFlat-mountDeafferentated Mouse RetinaRetinal NeurobiologyRetinal CircuitsWhole-cell RecordingNitrocellulose MembraneExternal SolutionCarbogenatedUpright Microscope

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Patch Clamp Recordings from Mouse Retinal Neurons in a Dark-adapted Slice Preparation

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells

Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

Whole-cell Patch-clamp Recordings in Brain Slices

Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

Read Article