/
/
Rápida de un solo paso Síntesis enzimática y All-Purificación acuosa de trehalosa Análogos
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Rápida de un solo paso Síntesis enzimática y All-Purificación acuosa de trehalosa Análogos
10,176 Views
•
09:27 min
•
February 17, 2017
DOI:
Summary
Automatically generated
Trehalose analogues are emerging as important molecules for bio(techno)logical and biomedical applications. We describe an optimized protocol for enzymatically synthesizing and purifying trehalose analogues that is simple, efficient, fast, and environmentally friendly. Its application to the rapid production and administration of a probe for the detection of mycobacteria is demonstrated.
Read Article
Cite this Article
Meints, L. M., Poston, A. W., Piligian, B. F., Olson, C. D., Badger, K. S., Woodruff, P. J., Swarts, B. M. Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues. J. Vis. Exp. (120), e54485, doi:10.3791/54485 (2017).
Copy