Rivista
/
Biologia dello sviluppo
/
Aggregate Size Optimization in Microputjes voor Suspension basis Cardiac Differentiatie van menselijke pluripotente stamcellen
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
Please note that all translations are automatically generated. Click here for the English version.

Aggregate Size Optimization in Microputjes voor Suspension basis Cardiac Differentiatie van menselijke pluripotente stamcellen

6,624 Views

•

06:28 min

•

September 25, 2016

DOI:

10.3791/54308-v

DOI:
10.3791/54308-v

•

06:28 min
September 25, 2016

•

1 Views
, ,
1Institute of Biomaterials and Biomedical Engineering,University of Toronto, 2Department of Comparative Biology and Experimental Medicine,University of Calgary

Summary

Automatically generated

Gebruikelijke werkwijzen tot inleiding suspensie geaggregeerde basis cardiale differentiatie van humane pluripotente stamcellen (hPSCs) geplaagd cultuur heterogeniteit met betrekking tot de totale grootte en vorm. Hier beschrijven we een robuuste methode voor cardiale differentiatie gebruik microputjes genereren maat gecontroleerde HPSC aggregaten gekweekt onder omstandigheden cardiale bevorderen.

Explore More Videos

Aggregate Size OptimizationMicrowellsSuspension-based Cardiac DifferentiationHuman Pluripotent Stem CellsCentrifugationCell SuspensionMicrowell PlateHypoxic IncubationAggregate HarvestingInduction Medium

Read Article

Cite this Article

Bauwens, C. L., Toms, D., Ungrin, M. Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells. J. Vis. Exp. (115), e54308, doi:10.3791/54308 (2016).

Download .ris file

Copy

Share Video

.

Copy