Journal
/
Biología del desarrollo
/
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
JoVE Revista
Biología del desarrollo
This content is Free Access.
JoVE Revista Biología del desarrollo
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells

Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells

DOI:
10.3791/54308-v

•

06:28 min

•

September 25, 2016

•

, ,
1Institute of Biomaterials and Biomedical Engineering,University of Toronto, 2Department of Comparative Biology and Experimental Medicine,University of Calgary

Capítulos

  • 00:05Título
  • 00:54Plating hPSC Aggregates in the Microwell Plate
  • 02:00Cardiac Induction Stage 1
  • 03:06Cardiac Induction Stage 2
  • 04:16Cardiac Induction Stage 3
  • 05:06Results: Characteristics of Differentiated hPSCs
  • 05:44Conclusion

Summary

Traducción Automática

Traducción Automática

Conventional methods to initiate suspension aggregate based cardiac differentiation of human pluripotent stems cells (hPSCs) are plagued with culture heterogeneity with respect to aggregate size and shape. Here, we describe a robust method for cardiac differentiation employing microwells to generate size-controlled hPSC aggregates cultured under cardiac-promoting conditions.

Tags

Aggregate Size OptimizationMicrowellsSuspension-based Cardiac DifferentiationHuman Pluripotent Stem CellsCentrifugationCell SuspensionMicrowell PlateHypoxic IncubationAggregate HarvestingInduction Medium

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Pluripotent Stem Cell Derived Cardiac Cells for Myocardial Repair

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Read Article