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Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells

Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells

DOI:
10.3791/54308-v

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06:28 min

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September 25, 2016

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, ,
1Institute of Biomaterials and Biomedical Engineering,University of Toronto, 2Department of Comparative Biology and Experimental Medicine,University of Calgary

Chapitres

  • 00:05Titre
  • 00:54Plating hPSC Aggregates in the Microwell Plate
  • 02:00Cardiac Induction Stage 1
  • 03:06Cardiac Induction Stage 2
  • 04:16Cardiac Induction Stage 3
  • 05:06Results: Characteristics of Differentiated hPSCs
  • 05:44Conclusion

Summary

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Conventional methods to initiate suspension aggregate based cardiac differentiation of human pluripotent stems cells (hPSCs) are plagued with culture heterogeneity with respect to aggregate size and shape. Here, we describe a robust method for cardiac differentiation employing microwells to generate size-controlled hPSC aggregates cultured under cardiac-promoting conditions.

Tags

Aggregate Size OptimizationMicrowellsSuspension-based Cardiac DifferentiationHuman Pluripotent Stem CellsCentrifugationCell SuspensionMicrowell PlateHypoxic IncubationAggregate HarvestingInduction Medium

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