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イメージングフローサイトメトリーによるヒト単球由来樹状細胞の特徴付け：二つの単球の分離プロトコルの比較
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
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イメージングフローサイトメトリーによるヒト単球由来樹状細胞の特徴付け：二つの単球の分離プロトコルの比較

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08:31 min

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October 18, 2016

DOI:

10.3791/54296-v

DOI:
10.3791/54296-v

•

08:31 min
October 18, 2016

•

3 Views
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1Department of Immunology, Herbert Wertheim College of Medicine,Florida International University, 2Millipore Sigma

Summary

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This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.

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MonocyteDendritic CellIsolationCharacterizationImaging Flow CytometryCell Surface ExpressionCD11cCD14PBMCAdherenceGMCSFIL4

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Cite this Article

Figueroa, G., Parira, T., Laverde, A., Casteleiro, G., El-Mabhouh, A., Nair, M., Agudelo, M. Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols. J. Vis. Exp. (116), e54296, doi:10.3791/54296 (2016).

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