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In vivo Calcium Imaging of Mouse Geniculate Ganglion Neuron Responses to Taste Stimuli
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In vivo Calcium Imaging of Mouse Geniculate Ganglion Neuron Responses to Taste Stimuli
Capitoli
- 00:05Introduction
- 00:38Tracheotomy
- 01:45Tympanic Bulla Opening
- 03:12Geniculate Exposure
- 04:34Tastant Panel Analysis
- 06:09Results: Representative Geniculate Ganglion Neuron Responses to Tastants Using In Vivo GCaMP6s Imaging
- 06:47Conclusion
Here we present how to expose the geniculate ganglion of a live, anesthetized laboratory mouse and how to use calcium imaging to measure the responses of ensembles of these neurons to taste stimuli, allowing for multiple trials with different stimulants. This allows for in depth comparisons of which neurons respond to which tastants.
