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Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain

Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain

10,898 Views

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10:09 min

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May 25, 2016

DOI:

10.3791/53635-v

DOI:
10.3791/53635-v

•

10:09 min
May 25, 2016

•

7 Views
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1Cell Biology,UMC Groningen, 2Clinical Genetics,Erasmus MC Rotterdam

Summary

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Large-scale 2D electron microscopy (EM), or nanotomy, is the tissue-wide application of nanoscale resolution EM. Here we describe a universal method for nanotomy applied to investigate the zebrafish larval brain in health and upon non-invasive brain injury.

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Cite this Article

Kuipers, J., Kalicharan, R. D., Wolters, A. H. G., van Ham, T. J., Giepmans, B. N. Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain. J. Vis. Exp. (111), e53635, doi:10.3791/53635 (2016).

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