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干细胞样<em>爪</em>胚胎外植体，研究早期神经发育特点<em>体外</em>和<em>在体内</em
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
Please note that all translations are automatically generated. Click here for the English version.

干细胞样<em>爪</em>胚胎外植体，研究早期神经发育特点<em>体外</em>和<em>在体内</em

7,774 Views

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11:13 min

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February 02, 2016

DOI:

10.3791/53474-v

DOI:
10.3791/53474-v

•

11:13 min
February 02, 2016

•

5 Views
1Institut Curie, 2UMR 3387,CNRS, 3PSL Research University, 4Université Paris-Sud

Summary

Automatically generated

In Xenopus embryos, cells from the roof of the blastocoel are pluripotent and can be programmed to generate various tissues. Here, we describe protocols to use amphibian blastocoel roof explants as an assay system to investigate key in vivo and in vitro features of early neural development.

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Cite this Article

Durand, B. C. Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo. J. Vis. Exp. (108), e53474, doi:10.3791/53474 (2016).

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