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JoVE Journal Developmental Biology
Imaging Subcellular Structures in the Living Zebrafish Embryo
 

Imaging Subcellular Structures in the Living Zebrafish Embryo

Article DOI: 10.3791/53456-v • 11:19 min • April 2nd, 2016
April 2nd, 2016 •
1, 1,2, 1,3, 1, 4,5,6, 1
1Institute of Neuronal Cell Biology, Technische Universität München, 2Cell Biology, Department of Biology, Faculty of Science, Utrecht University, 3Faculty of Biology, Ludwig-Maximilians-Universität-München, 4Adolf-Butenandt-Institute, Biochemistry, Ludwig-Maximilians-Universität-München, 5German Center for Neurodegenerative Diseases, 6Laboratory of Brain Development and Repair, The Rockefeller University

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Imaging the dynamic behavior of organelles and other subcellular structures in vivo can shed light on their function in physiological and disease conditions. Here, we present methods for genetically tagging two organelles, centrosomes and mitochondria, and imaging their dynamics in living zebrafish embryos using wide-field and confocal microscopy.

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Imaging Method Subcellular Structures Living Zebrafish Embryo Vertebrate In Vivo Neuronal Cell Biology Dynamics Of Mitochondria Centrosomes Intact Vertebrate Surgical Procedure Physiological Conditions Alzheimer's Disease Microinjection Stable Transgenic Lines Danieau's Solution PTU Pigment Formation Transgene Expression Fluorescence Dissecting Microscope Anesthetized Embryos Petri Dish Tricaine Low-melting Agarose
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