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마우스 비장에서 불변 자연 살해 T 세포를 분리하고 확장을위한 최적화 방법
Capitoli
- 00:05Titolo
- 01:06Enrichment, Detection and Purification of Mouse Invariant Natural Killer T (iNKT) Cells
- 04:55Mouse iNKT Cell Culture and Expansion
- 07:13Results: Absolute Number and Cytokine Producing Capacity of In Vitro Expanded iNKT Cells
- 08:30Conclusion
Here we present an adapted protocol that can be used to generate a large number of murine invariant natural killer T cells from mouse spleen. The protocol outlines an approach by which splenic iNKT cells can be enriched for, isolated and expanded in vitro using a limited number of animals and reagents.
