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الحث المباشر للمكون للدم البطانة Endothelium والدم عن طريق overexpression من العوامل المحفزة النسخ في الإنسان الخلايا الجذعية
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
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الحث المباشر للمكون للدم البطانة Endothelium والدم عن طريق overexpression من العوامل المحفزة النسخ في الإنسان الخلايا الجذعية

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08:14 min

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December 03, 2015

DOI:

10.3791/52910-v

DOI:
10.3791/52910-v

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08:14 min
December 03, 2015

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1 Views
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1Primate Research Center,University of Wisconsin-Madison, 2Department of Pathology and Laboratory Medicine,University of Wisconsin School of Medicine and Public Health

Summary

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This protocol describes the efficient induction of hemogenic endothelium and multipotential hematopoietic progenitors from human pluripotent stem cells via the forced expression of transcription factors.

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Hemogenic EndotheliumHematopoietic CellsPluripotent Stem CellsTranscription FactorsETV2GATA1GATA2TAL1LMO2Endothelial-hematopoietic TransitionErythroidMegakaryocyticIn Vitro Modeling

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Cite this Article

Elcheva, I., Brok-Volchanskaya, V., Slukvin, I. Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells. J. Vis. Exp. (106), e52910, doi:10.3791/52910 (2015).

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