This protocol describes the efficient induction of hemogenic endothelium and multipotential hematopoietic progenitors from human pluripotent stem cells via the forced expression of transcription factors.
Elcheva, I., Brok-Volchanskaya, V., Slukvin, I. Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells. J. Vis. Exp. (106), e52910, doi:10.3791/52910 (2015).