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쥐 복막 대 식세포의 분리는 수신자 같은 수용체 자극하면 유전자 발현 분석을 수행하기
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
Please note that all translations are automatically generated. Click here for the English version.

쥐 복막 대 식세포의 분리는 수신자 같은 수용체 자극하면 유전자 발현 분석을 수행하기

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08:21 min

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April 29, 2015

DOI:

10.3791/52749-v

DOI:
10.3791/52749-v

•

08:21 min
April 29, 2015

•

36 Views
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1Centre de recherche du Centre hospitalier de l’Université de Montréal,Institut du cancer de Montréal, 2Département de Médecine,Université de Montréal

Summary

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We describe here a simple protocol to isolate murine peritoneal macrophages. This procedure is followed by RNA extraction to carry out gene expression analysis upon Toll-like receptors stimulation.

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Cite this Article

Layoun, A., Samba, M., Santos, M. M. Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation. J. Vis. Exp. (98), e52749, doi:10.3791/52749 (2015).

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