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In Vivo Assessment of Rodent Plasmodium Parasitemia and Merozoite Invasion by Flow Cytometry
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Capitoli
- 00:05Titolo
- 01:11Red Blood Cell Labeling and Transfusion
- 03:07Blood Sample Collection and Preparation for Flow Cytometry
- 04:10Flow Cytometry
- 05:19Results: Parasitemia Measurement and Merozoite Invasion Assessment
- 06:32Conclusion
The malaria parasite invades and replicates within red blood cells. The accurate assessment of merozoite invasion and parasitemia is therefore crucial in assessing the course of malaria infection. Here we describe a flow cytometry based protocol for the measurement of these parameters in a mouse model of malaria.
