This content is Free Access.
表面增强拉曼光谱检测生物分子利用EBL装配纳米衬底
Capitoli
- 00:05Titolo
- 02:04Fabrication of Nano-patterned PMMA Masks Using Electron Beam Lithography (EBL)
- 03:47Noble Metal Nanostructures Fabrication
- 04:41Bio-functionalization of Substrates
- 08:14Raman Spectroscopy
- 09:09Results: Nanostructure-enabled SERS Spectra of Biopolymers
- 10:29Conclusion
We describe the fabrication and characterization of nano-biological systems interfacing nanostructured substrates with immobilized proteins and aptamers. The relevant experimental steps involving lithographic fabrication of nanostructured substrates, bio-functionalization, and surface-enhanced Raman spectroscopy (SERS) characterization, are reported. SERS detection of surface-immobilized proteins, and probing of protein-ligand and aptamer-ligand binding is demonstrated.
