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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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Ingenieurwesen
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JoVE Journal Ingenieurwesen
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

19,842 Views

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11:44 min

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March 20, 2015

DOI:

10.3791/52712-v

DOI:
10.3791/52712-v

•

11:44 min
March 20, 2015

•

8 Views
, , ,
1Department of Electrical and Computer Engineering,University of Alberta, 2National Institute for Nanotechnology,National Research Council of Canada

Summary

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We describe the fabrication and characterization of nano-biological systems interfacing nanostructured substrates with immobilized proteins and aptamers. The relevant experimental steps involving lithographic fabrication of nanostructured substrates, bio-functionalization, and surface-enhanced Raman spectroscopy (SERS) characterization, are reported. SERS detection of surface-immobilized proteins, and probing of protein-ligand and aptamer-ligand binding is demonstrated.

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Peters, R. F., Gutierrez-Rivera, L., Dew, S. K., Stepanova, M. Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates. J. Vis. Exp. (97), e52712, doi:10.3791/52712 (2015).

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