Rivista
/
Ingegneria
/
표면 EBL 날조 된 나노 구조 기판을 사용하여 생체 분자의 라만 분광학 감지 강화
JoVE Journal
Ingegneria
This content is Free Access.
JoVE Journal Ingegneria
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Please note that all translations are automatically generated. Click here for the English version.

표면 EBL 날조 된 나노 구조 기판을 사용하여 생체 분자의 라만 분광학 감지 강화

19,833 Views

•

11:44 min

•

March 20, 2015

DOI:

10.3791/52712-v

DOI:
10.3791/52712-v

•

11:44 min
March 20, 2015

•

8 Views
, , ,
1Department of Electrical and Computer Engineering,University of Alberta, 2National Institute for Nanotechnology,National Research Council of Canada

Summary

Automatically generated

We describe the fabrication and characterization of nano-biological systems interfacing nanostructured substrates with immobilized proteins and aptamers. The relevant experimental steps involving lithographic fabrication of nanostructured substrates, bio-functionalization, and surface-enhanced Raman spectroscopy (SERS) characterization, are reported. SERS detection of surface-immobilized proteins, and probing of protein-ligand and aptamer-ligand binding is demonstrated.

Explore More Videos

Surface Enhanced Raman SpectroscopySERSNanostructured SubstratesElectron Beam LithographyBiomolecule ImmobilizationProtein AGlucose Binding ProteinDopamine Binding DNA AptamerProtein-ligand BindingAptamer-ligand Binding

Read Article

Cite this Article

Peters, R. F., Gutierrez-Rivera, L., Dew, S. K., Stepanova, M. Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates. J. Vis. Exp. (97), e52712, doi:10.3791/52712 (2015).

Download .ris file

Copy

Share Video

.

Copy