Rivista
/
Immunologia e infezioni
/
Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

DOI:
10.3791/52687-v

•

09:43 min

•

April 16, 2015

•

, , , ,
1LD MacLean Surgical Research Laboratories, Department of Surgery,McGill University

Capitoli

  • 00:05Titolo
  • 01:14Neutrophil Isolation from Whole Blood
  • 03:38Neutrophil Extracellular Trap (NET) Generation and Isolation
  • 05:24NET-cancer Cell Static Adhesion Assay
  • 08:18Results: Simple Generation and Isolation of NETs from Human Whole Blood
  • 09:12Conclusion

Summary

Traduzione automatica

Traduzione automatica

In the following protocol, we describe a very simple way to isolate Neutrophil Extracellular traps (NETs) from human whole blood using readily available reagents. We then demonstrate how the isolated NETs can be used in an in vitro adhesion assay with cancer cells.

Tags

Neutrophil Extracellular Traps (NETs)NET IsolationNET HandlingCell-free NET IsolationNET-based AssaysNET Adhesion AssayA549 Lung Cancer CellsPMA StimulationDensity Gradient SeparationDNase1

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Neutrophil Extracellular Traps: How to Generate and Visualize Them

Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT

Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA

Author Spotlight: Neutrophil Extracellular Traps Imaging in Human and Mouse Tissues

Author Spotlight: Quantifying Neutrophil Extracellular Traps in Disease and Drug Screening Using Dual-Color Live-Cell Imaging

Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples

Read Article