Rivista
/
Neuroscienze
/
Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

DOI:
10.3791/51863-v

•

10:15 min

•

September 15, 2014

•

, , , ,
1Department of Neuroscience,Baylor College of Medicine, 2Department of Neuroscience, Center for Translational Research in Neurodegenerative Disease,University of Florida, 3Department of Neurology and Department of Neurosurgery,Baylor College of Medicine

Capitoli

  • 00:05Titolo
  • 01:24Staging the Newborn Pups for Injection
  • 02:38Free-hand Intracranial Injection of AAV into Neonatal Mice
  • 04:54Stereotaxic Injection of AAV into Neonatal Mice
  • 07:21Post-injection Care
  • 07:54Effect of Viral Titer and Serotype on VIral Transduction and Co-expression
  • 09:55Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we demonstrate a technique for widespread neuronal transduction by intraventricular injection of adeno-associated virus into the neonatal mouse brain. This method provides a rapid and easy way to attain lifelong expression of virally-delivered transgenes.

Tags

IntracerebroventricularViral InjectionNeonatal Mouse BrainNeuronal TransductionAdeno-associated VirusAAVEpendymal LiningGene ExpressionFluorescent ProteinViral TiterStereotaxic Frame

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain

Neurobehavioral Assessments in a Mouse Model of Neonatal Hypoxic-ischemic Brain Injury

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery

Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging

Analyzing the Parkinson’s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy

Read Article