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영구와 광범위한 신경 세포의 형질 도입에 대한 신생아 마우스 뇌의 뇌실 바이러스 감염
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Neuroscienze
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JoVE Journal Neuroscienze
Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction
Please note that all translations are automatically generated. Click here for the English version.

영구와 광범위한 신경 세포의 형질 도입에 대한 신생아 마우스 뇌의 뇌실 바이러스 감염

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10:15 min

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September 15, 2014

DOI:

10.3791/51863-v

DOI:
10.3791/51863-v

•

10:15 min
September 15, 2014

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82 Views
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1Department of Neuroscience,Baylor College of Medicine, 2Department of Neuroscience, Center for Translational Research in Neurodegenerative Disease,University of Florida, 3Department of Neurology and Department of Neurosurgery,Baylor College of Medicine

Summary

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Here we demonstrate a technique for widespread neuronal transduction by intraventricular injection of adeno-associated virus into the neonatal mouse brain. This method provides a rapid and easy way to attain lifelong expression of virally-delivered transgenes.

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IntracerebroventricularViral InjectionNeonatal Mouse BrainNeuronal TransductionAdeno-associated VirusAAVEpendymal LiningGene ExpressionFluorescent ProteinViral TiterStereotaxic Frame

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Kim, J., Grunke, S. D., Levites, Y., Golde, T. E., Jankowsky, J. L. Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction. J. Vis. Exp. (91), e51863, doi:10.3791/51863 (2014).

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