Rivista
/
Bioingegneria
/
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)
JoVE Journal
Bioingegneria
This content is Free Access.
JoVE Journal Bioingegneria
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

DOI:
10.3791/51549-v

•

07:04 min

•

May 23, 2014

•

, , , , , , , , ,
1Plate-Forme d'Imagerie Dynamique, Imagopole,Institut Pasteur, 2Department of Radiation Oncology,Stanford School of Medicine, 3Service Hospitalier Frédéric Joliot,Institut d'Imagerie Biomédicale, 4Vanderbilt School of Medicine, 5The Walter & Eliza Hall Institute of Medical Research, 6Unité INSERM U786,Institut Pasteur, 7Unité de Pathogénie Microbienne Moléculaire,Institut Pasteur

Capitoli

  • 00:05Titolo
  • 01:09Basic Recapitulation of FUEL
  • 02:32FUEL Over Varying Distances
  • 04:49Investigating Potential FUEL Pairs
  • 05:53Results: Analysis of the Various FUEL Demonstrations
  • 06:40Conclusion

Summary

Traduzione automatica

Traduzione automatica

Expanding the foundation and applicability of Fluorescence by Unbound Excitation from Luminescence (FUEL) by surveying the relevant principles and demonstrating its compatibility with a multitude of fluorophores and antibody-targeted conditions.

Tags

FUELFluorescenceLuminescenceBRETRadiative ExcitationEnergy TransferFluorophoresFluorescent NanoparticlesAntibody-targeted FUELIn VitroIn VivoWhole Animal Imaging

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Engineering ‘Golden’ Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

Read Article