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A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)
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May 23, 2014
DOI:
1Plate-Forme d'Imagerie Dynamique, Imagopole,Institut Pasteur, 2Department of Radiation Oncology,Stanford School of Medicine, 3Service Hospitalier Frédéric Joliot,Institut d'Imagerie Biomédicale, 4Vanderbilt School of Medicine, 5The Walter & Eliza Hall Institute of Medical Research, 6Unité INSERM U786,Institut Pasteur, 7Unité de Pathogénie Microbienne Moléculaire,Institut Pasteur
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Expanding the foundation and applicability of Fluorescence by Unbound Excitation from Luminescence (FUEL) by surveying the relevant principles and demonstrating its compatibility with a multitude of fluorophores and antibody-targeted conditions.
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Dragavon, J., Sinow, C., Holland, A. D., Rekiki, A., Theodorou, I., Samson, C., Blazquez, S., Rogers, K. L., Tournebize, R., Shorte, S. L. A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL). J. Vis. Exp. (87), e51549, doi:10.3791/51549 (2014).
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