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Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey
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Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey
Capitoli
- 00:05Titolo
- 01:22Preparing the Larval Sea Lamprey
- 02:17Complete Spinal Cord Transection
- 04:30Brain Dissection
- 06:55Results: Expression of neogenin After Spinal Cord Transection
- 07:35Conclusion
Lampreys recover locomotion after a complete spinal cord injury. However, some spinal-projecting neurons are good regenerators and others are not. This paper illustrates the techniques for housing sea lamprey larvae (and recently transformed adults), producing complete spinal cord transections and preparing wholemount brains and spinal cords for in situ hybridization.
