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Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey
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Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey

Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey

9,747 Views

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08:11 min

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October 14, 2014

DOI:

10.3791/51494-v

DOI:
10.3791/51494-v

•

08:11 min
October 14, 2014

•

1 Views
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1Centre for Neuroregeneration, School of Biomedical Sciences,University of Edinburgh, 2Shriners Hospitals Pediatric Research Center (Center for Neural Repair and Rehabilitation),Temple University School of Medicine, 3Department of Neurology,Temple University School of Medicine

Tags

Spinal Cord InjuryRegenerationWholemount In Situ HybridizationLarval Sea LampreyBrainstem NeuronsPropriospinal AxonsAxon RegenerationNeuron-intrinsic MechanismsGene ExpressionComplete TransectionHousingAnesthesiaDissection Protocol

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Barreiro-Iglesias, A., Zhang, G., Selzer, M. E., Shifman, M. I. Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey. J. Vis. Exp. (92), e51494, doi:10.3791/51494 (2014).

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