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08:11 min
October 14, 2014
DOI:
10.3791/51494-v
七鳃鳗完整的脊髓损伤后恢复运动。然而,一些脊髓投射神经元是很好的再生和其他人都没有。本文说明了技术的外壳海七鳃鳗幼体(最近转化成人),产生完全性脊髓横断和原位杂交准备wholemount大脑和脊髓的。
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Barreiro-Iglesias, A., Zhang, G., Selzer, M. E., Shifman, M. I. Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey. J. Vis. Exp. (92), e51494, doi:10.3791/51494 (2014).
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