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Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
Capitoli
- 00:05Titolo
- 02:14Cell Culture and Treatment
- 04:58Click-iT Assay
- 09:59Light Microscopy and Data Analysis
- 12:41Results: RNA Expression in Hypoxic Cells
- 14:02Conclusion
We describe a technique for analysis of global RNA synthesis in hypoxia using imaging. Click-chemistry labeling of RNA has not previously been performed under hypoxia and allows visualization of global RNA changes at the single cell level. This approach complements the existing averaged RNA techniques, allowing direct visualization of cell-to-cell changes in global RNA synthesis.
