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Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
 

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Article DOI: 10.3791/51420-v • 14:53 min • May 13th, 2014
May 13th, 2014 •
1, 1, 1, 1, 1, 1
1Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, UK

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We describe a technique for analysis of global RNA synthesis in hypoxia using imaging. Click-chemistry labeling of RNA has not previously been performed under hypoxia and allows visualization of global RNA changes at the single cell level. This approach complements the existing averaged RNA techniques, allowing direct visualization of cell-to-cell changes in global RNA synthesis.

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RNA Synthesis Single Cell Analysis Hypoxia Oxygen Availability Transcriptional Program Cellular Response Oxygen Sensor Enzymes Prolyl-hydroxylases Histone Demethylases Cellular Populations Average Studies Click-iT RNA Imaging Kits Oxygen Controlled Workstation Microscopy Analysis Quantification Cancer Cells Temporal Changes Cell-to-cell Changes
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