Rivista
/
Immunologia e infezioni
/
Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

10,511 Views

•

12:47 min

•

November 03, 2014

DOI:

10.3791/51306-v

DOI:
10.3791/51306-v

•

12:47 min
November 03, 2014

•

15 Views
, ,
1Integrated Department of Immunology and Integrated Center for Genes, Environment, and Health,National Jewish Health and University of Colorado School of Medicine

Summary

Automatically generated

In this protocol, we describe methods to efficiently transfect murine macrophage cell lines with siRNAs using the Amaxa Nucleofector 96-well Shuttle System, stimulate the macrophages with lipopolysaccharide, and monitor the effects on inflammatory cytokine production.

Explore More Videos

RNA InterferenceMacrophageInnate Immune ResponseGene KnockdownSiRNA TransfectionAmaxa Nucleofector96-well FormatLipopolysaccharideCytokine Production

Read Article

Cite this Article

De Arras, L., Guthrie, B. S., Alper, S. Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages. J. Vis. Exp. (93), e51306, doi:10.3791/51306 (2014).

Download .ris file

Copy

Share Video

.

Copy