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Immunology and Infection

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JoVE Journal Immunology and Infection
Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
 

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Article DOI: 10.3791/51306-v • 12:47 min • November 3rd, 2014
November 3rd, 2014 •
1, 1, 1
1Integrated Department of Immunology and Integrated Center for Genes, Environment, and Health, National Jewish Health and University of Colorado School of Medicine

Kapitel

Summary
Automatic Translation

In this protocol, we describe methods to efficiently transfect murine macrophage cell lines with siRNAs using the Amaxa Nucleofector 96-well Shuttle System, stimulate the macrophages with lipopolysaccharide, and monitor the effects on inflammatory cytokine production.

Tags

RNA-interference Innate Immune Response Mouse Macrophages Antimicrobial Proteins Chemokines Cytokines Adaptive Immune Response RNAi Gene Knockdown Transfection Amaxa Nucleofector 96-well Shuttle System SiRNA Lipopolysaccharide (LPS)-induced Cytokine Production
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