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High-throughput Screening for Bredspektret Chemical hæmmere af RNA virus
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High-throughput Screening for Bredspektret Chemical hæmmere af RNA virus
Capitoli
- 00:05Titolo
- 01:34Preparation of 96-well Daughter Plates with Compounds
- 03:03Preparation of Cell Cultures to Determine Compound Toxicity
- 04:18Preparation of Cell Cultures Infected by rMV2/Luc
- 05:37Luciferase Activity Measurement
- 06:36Secondary Screening for Toxicity and Broad-spectrum Antiviral Activity
- 08:52Results: Identification of Anti-viral Hit Compounds
- 11:06Conclusion
In vitro assays til at måle virus replikation er blevet kraftigt forbedret gennem udvikling af rekombinante RNA-virus, der udtrykker luciferase eller andre enzymer, der kan bioluminescens. Her har vi detalje en high-throughput screening rørledning, der kombinerer disse rekombinante stammer af mæslinger og Chikungunya virus at isolere bredspektrede antivirale fra kemiske biblioteker.
