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Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
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Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
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December 17, 2013
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This article illustrates the use of pulse-chase radio labeling in combination with site-specific photocrosslinking to monitor interactions between a protein of interest and other factors in E. coli. Unlike traditional chemical cross-linking methods, this approach generates high resolution “snapshots” of an ordered assembly pathway in a living cell.
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Pavlova, O., Ieva, R., Bernstein, H. D. Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking. J. Vis. Exp. (82), e51217, doi:10.3791/51217 (2013).
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