Journal
/
/
Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

6,055 Views

11:33 min

December 17, 2013

DOI:

11:33 min
December 17, 2013

2 Views
, ,

Summary

Automatically generated

This article illustrates the use of pulse-chase radio labeling in combination with site-specific photocrosslinking to monitor interactions between a protein of interest and other factors in E. coli. Unlike traditional chemical cross-linking methods, this approach generates high resolution “snapshots” of an ordered assembly pathway in a living cell.

Read Article