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Anti-nucleaire antilichaam screening met HEp-2 cellen
JoVE Journal
Biologia
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JoVE Journal Biologia
Anti-Nuclear Antibody Screening Using HEp-2 Cells
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Anti-nucleaire antilichaam screening met HEp-2 cellen

132,412 Views

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13:01 min

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June 23, 2014

DOI:

10.3791/51211-v

DOI:
10.3791/51211-v

•

13:01 min
June 23, 2014

•

39 Views
, , ,
1IFA Development Manager,INOVA Diagnostics, Inc., 2IFA Development,INOVA Diagnostics, Inc., 3Product Manager, Rheumatology,INOVA Diagnostics, Inc., 4Medical Director,INOVA Diagnostics, Inc.

Summary

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Indirecte immunofluorescentie (IIF) assays traditioneel gebruikt voor de detectie van antinucleaire antilichamen (ANA) in menselijk serum. De aanwezigheid van deze antilichamen kunnen helpen bij de diagnose van systemische auto reumatische aandoeningen (SARD). Dit protocol laat zien hoe effectief uit te voeren de IIF techniek om deze autoantilichamen nauwkeurig te detecteren.

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Cite this Article

Buchner, C., Bryant, C., Eslami, A., Lakos, G. Anti-Nuclear Antibody Screening Using HEp-2 Cells. J. Vis. Exp. (88), e51211, doi:10.3791/51211 (2014).

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