Rivista
/
Immunologia e infezioni
/
隔离和幼稚的CD4 T细胞的Th17细胞分化
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Please note that all translations are automatically generated. Click here for the English version.

隔离和幼稚的CD4 T细胞的Th17细胞分化

DOI:
10.3791/50765-v

•

12:59 min

•

September 26, 2013

•

, , , ,
1Department of Microbiology and Cell Science,The University of Florida

Capitoli

  • 00:05Titolo
  • 01:27Plate-bound anti-CD3
  • 02:03Mouse Dissection
  • 04:40Th17 Differentiation
  • 05:40qPCR and ELISA
  • 06:27Cell Activation
  • 07:09Intracellular Staining
  • 08:55Flow Cytometric Analysis
  • 09:24Results: Isolation and Differentiation of Th17 Cells
  • 12:14Conclusion

Summary

Traduzione automatica

Traduzione automatica

与效应器功能从其它的T细胞亚群的不同的，Th17细胞已被集中牵连的炎性自身免疫性疾病。这种体外 Th17细胞分化协议提供了一种方法来确定初始CD4 + T淋巴细胞是否能够分化成Th17细胞，并进一步研究其在自身免疫和宿主反应的作用。

Tags

Th17 CellsCD4+ T LymphocytesAutoimmune DisordersIL-17T Cell DifferentiationFlow CytometryQPCRELISA

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

β细胞死亡介导细胞毒性T淋巴细胞的方法来评估

定向分化诱导性多潜能干细胞向T淋巴细胞

小鼠生殖道黏膜淋巴细胞分离

腺病毒转导朴素的CD4 T细胞调节性T细胞分化研究

尾部皮肤来研究异基因CD4 + T细胞应答的小鼠移植

鼠标朴素CD4<sup> +</sup> T细胞分离和<em>在体外</em>分化为T细胞亚群

从人类腭扁桃体B和T淋巴细胞有效隔离协议

分离和<em>离体</emVδ1的>企业文化<sup> +</sup> CD4<sup> +</sup>γδT细胞，胸腺外的αβT细胞祖

成像的CD4 T细胞间质迁移发炎真皮

分离及小鼠淋巴细胞的激活

Read Article