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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

9,421 Views

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14:44 min

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December 16, 2013

DOI:

10.3791/50747-v

DOI:
10.3791/50747-v

•

14:44 min
December 16, 2013

•

6 Views
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1Department of Chemistry,The Hebrew University of Jerusalem, 2Wolfson Centre for Applied Structural Biology,The Hebrew University of Jerusalem

Summary

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The NMR-solution structure of a metallochaperone model peptide with Cu (I) was determined, and a detailed protocol from sample preparation and 1D and 2D data collection to a three-dimensional structure is described.

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Peptide-metal ComplexesCopper (I) BindingMetallochaperone Transport ProteinsNMR Spectroscopy1D And 2D 1H NMRStructure DeterminationMolecular Mechanics CalculationsSolution StructureUnbiased Structure Determination

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Shoshan, M. S., Tshuva, E. Y., Shalev, D. E. Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR. J. Vis. Exp. (82), e50747, doi:10.3791/50747 (2013).

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