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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
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化学
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JoVE 杂志 化学
Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

DOI:
10.3791/50747-v

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14:44 min

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December 16, 2013

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, ,
1Department of Chemistry,The Hebrew University of Jerusalem, 2Wolfson Centre for Applied Structural Biology,The Hebrew University of Jerusalem

Chapters

  • 00:05Title
  • 01:35Sample Preparation
  • 02:45NMR Data Collection and Processing
  • 03:50Peak Assignment and Integration Using SPARKY
  • 05:07Molecular Mechanics Calculations to Generate Structure Ensemble Using XPLOR
  • 08:07Structure Analysis
  • 10:39Results: Structural Analysis of a Metallochaperone Protein-mimetic Peptide-copper Complex
  • 14:04Conclusion

Summary

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The NMR-solution structure of a metallochaperone model peptide with Cu (I) was determined, and a detailed protocol from sample preparation and 1D and 2D data collection to a three-dimensional structure is described.

Tags

Peptide-metal ComplexesCopper (I) BindingMetallochaperone Transport ProteinsNMR Spectroscopy1D And 2D 1H NMRStructure DeterminationMolecular Mechanics CalculationsSolution StructureUnbiased Structure Determination

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