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对于电感受的制备快速协议<em>大肠杆菌</em>和<em>霍乱弧菌</em

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae

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对于电感受的制备快速协议<em>大肠杆菌</em>和<em>霍乱弧菌</em

74,988 Views

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07:10 min

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October 08, 2013

DOI:

10.3791/50684-v

DOI:

10.3791/50684-v

•

07:10 min

October 08, 2013

•

37 Views

Miguel F. Gonzales¹, Teresa Brooks², Stefan U. Pukatzki², Daniele Provenzano^1,3

¹Department of Biological Sciences,University of Texas Brownsville, ²Department of Medical Microbiology and Immunology,University of Alberta, ³Department of Biomedical Sciences,University of Texas Brownsville

Summary

电穿孔是用于将DNA导入细菌中称为转化的过程中通常采用的方法。为电感受态细胞的制备传统的协议是耗时且劳动密集型的。本文介绍了一种替代，快速，并为电感受态细胞通过一些实验室目前使用的高效制备方法。

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